Maximum likelihood tree of subgenus Simulium. Full view of the tree is in the top left corner. The bootstrap value and Bayesian Inference (BI) are shown on the branches. Sequences generated from this study are in bold. Vertical bars on the right are the result of species delimitation, with the species group indicated to the right. Species with an asterisk (*) came from a different species group: Simulium kiuliense belongs to the S. nobile species group but was assigned to the S. melanopus species group by species delimitation.