Research Article
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Article title
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Key words
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1. Introduction
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2. Methods
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2.1. Mosquito collection and morphological identification
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2.2. DNA extraction, amplification, and sequencing
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2.3. Sequencing Alignment and Phylogenetic Analysis
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3. Results
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3.1. Morphological characteristics of females
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3.1.1. Aedes impiger
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3.1.2. Aedes nigripes
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3.1.3. Aedes hexodontus
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3.1.4. Aedes punctor
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3.1.5. Punctor subgroup’s outlier
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3.2. Phylogenetic analysis
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4. Discussion
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4.1. The tarsal claw and the COI barcoding region can differentiate Aedes impiger and Aedes nigripes
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4.2. Aedes hexodontus and Aedes punctor are not reliably differentiated and may not warrant separate species status
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4.3. Challenges with sequences on public databases: underrepresentation, inaccurate identifications, and overbinning
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4.4. Urgent needs for baselines on vectors and vector-borne diseases in the Arctic as the global hotspot of climate change
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5. Conclusion
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6. Funding
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7. Competing interests
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8. Author’s contributions
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9. Acknowledgements
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10. References
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