Simplified phylogram from Maximum Likelihood analysis using COI dataset from current study, using public sequences (with their respective GenBank number) and sequences from our own specimens (in colour). Sequences not partitioned per codon position. Results show one polyphyletic group, regrouping Aedes hexodontus (in orange), Aedes punctor (in red) and Punctor subgroup’s outliers (in magenta), and two monophyletic groups, Aedes impiger (in green) and Aedes nigripes (in blue). Mansonia uniformis used as outgroup. Bootstrap values from Maximum likelihood analysis (in bold) and posterior probabilities from Bayesian inference analysis (in bold, underlined) displayed as support values. Five support values chosen (A to E, in yellow highlight) and compared with same dataset partitioned by codons. Table indicates superior performance of unpartitioned sequence data (higher support in green) in comparison to sequence data portioned per codon position. Sampling regions in parentheses: AK (Alaska, USA); ON (Ontario, CAN); NU (Nunavut, CAN); QC (Québec, CAN); MN (Manitoba, CAN); NL (Newfoundland and Labrador, CAN).