Research Article
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Article title
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Abstract
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Key words
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1. Introduction
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2. Material and methods
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2.1. Material examined
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2.2. Morphological analyses
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2.2.1. Measurements
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2.2.2. Procedure for preparation of male terminalia
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2.2.3. Photography and preparation of the figures
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2.3. Molecular analyses
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2.3.1. Mitochondrial DNA
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2.3.2. Nuclear DNA
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2.4. Analyses of phylogeny
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2.4.1. Partitioning
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2.4.2. Phylogeny estimation
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3. Results
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3.1. Analysis of field data
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3.2. Analysis of morphological data
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3.3. Analysis of molecular data
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3.3.1. Mitochondrial genetic distances
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3.3.2. Phylogenetic analysis
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4. Discussion
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4.1. Ecotype formation event
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4.2. Ecotype versus subspecies
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4.3. Recognition of ecotypic variations in Anoplistes
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4.4. An evolutionary scenario of speciation
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4.5. Environmental influence
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4.6. Analysis of relationships among closely related genera of Trachyderini
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5. Authors’ contributions
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6. Acknowledgements
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7. References