Mitochondrial chronogram based on the ND2 phylogenetic reconstruction of the Poecilimon ampliatus species group. Blue line indicates climate fluctuations over time (by Robert A. Rohde, based on data from Petit et al. 1999; Lisiecki and Raymo 2005). Blue arrow indicates the switch from 41 to 100 kyr glacials. Red arrow indicates the Mid-Brunhes Transition. Black numbers before the nodes correspond to time estimation in millions of years. Numbers in white circles at the branches refer to Supplementary material 2, where they correspond to the 95% HPD intervals.