Locus;Primers;"Sequence (5’-3’)";"Size (bp)";"PCR conditions"
COI;LCO1490;GGTCAACAAATCATAAAGATATTGG;~710;"3mM MgCl, 0.4μM primers, 0.2mM dNTP’s, 1U Taq DNA polymerase"
HCO2198;TAAACTTCAGGGTGACCAAAAAATCA;"(94oC/1min, 42-50oC/1min, 72oC/1min) 35 cycles"
C1J1632;TGATCAAATTTATAAT;~560;"3mM MgCl, 0.4μM primers, 0.2mM dNTP’s, 1U Taq DNA polymerase"
C1N2191;GGTAAAATTAAAATATAAACTTC;"(94oC/1min, 42oC/1min, 72oC/1min) 35 cycles"
CytB;CB3-PDR;CAYATTCAACCWGAATGATA;~550;"3mM MgCl, 0.6μM primers, 0.2mM dNTP’s, 1U Taq DNA polymerase"
N1N-PDR;GGCAYWTTGCCTCGAWTTCGWTATGA;"(94oC/1min, 43-46oC/1min, 72oC/1min) 35 cycles"
ITS2;C1a;CCTGGTTAGTTTCTTTTCCTCCGCT;~530;"2.5mM MgCl, 0.6μM primers, 0.2mM dNTP’s, 1.5U Taq DNA polymerase"
JTS3;CGCAGCTAACTGTGTGAAATC;"(94oC/1min, 54-64oC/1min, 72oC/1min) 35 cycles"
28S;rc28H;CTACTATCCAGCGAAACC;~680;"2mM MgCl, 0.6μM primers, 0.2mM dNTP’s, 1.5U Taq DNA polymerase"
28K;GAAGAGCCGACATCGAAG;"Touchdown PCR (94oC/1min, 60+58oC/1min, 72oC/1min) 5+32 cycles"
EF1-α;EF-F05;CCTGGACATCGTGATTTCAT;~500;"2.5mM MgCl, 0.6μM primers, 0.2mM dNTP’s, 1.5U Taq DNA polymerase"
EF-R08;CCACCAATCTTGTAGACATCCTG;"(94oC/0.5min, 44-48oC/0.5min, 72oC/0.5min) 35 cycles"
TPI;TPI111Fb;GGNAAYTGGAARATGAAYGG;~460;"3mM MgCl, 0.6μM primers, 0.2mM dNTP’s, 1.5U Taq DNA polymerase"
TPI275R;GCCCANACNGGYTCRTANGC;"Touchdown PCR (94oC/0.5min, 54+50+45oC/0.5min, 72oC/1.5min) 5+5+30 cycles"