Research Article |
Corresponding author: Oldrich Sychra ( sychrao@vfu.cz ) Academic editor: Christiane Weirauch
© 2021 Oldrich Sychra, Stanislav Kolencik, Ivo Papousek, Branka Bilbija, Ivan Literak.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Sychra O, Kolencik S, Papousek I, Bilbija B, Literak I (2021) Myrsidea quadrifasciata (Phthiraptera: Amblycera) – a unique host generalist among highly host-specific chewing lice. Arthropod Systematics & Phylogeny 79: 379-400. https://doi.org/10.3897/asp.79.e63975
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Abstract
Ten species of the louse genus Myrsidea belonging to the “serini-species-group” have been reviewed. A redescription of Myrsidea quadrifasciata (Piaget, 1880), the earliest described and valid species of this species complex, is given and a neotype for this species is designated. Nine new junior synonymies of M. quadrifasciata are proposed and discussed. The new synonyms and their respective type hosts are: Myrsidea anoxanthi Price and Dalgleish, 2007 from Loxipasser anoxanthus (Gosse, 1847), Myrsidea argentina (Kellogg, 1906) from Spinus magellanicus (Vieillot, 1805), Myrsidea balati Macháček, 1977 from Passer montanus (Linnaeus, 1758), Myrsidea darwini Palma and Price, 2010 from Geospiza fuliginosa Gould, 1837, Myrsidea major (Piaget, 1880) from Plectrophenax nivalis (Linnaeus, 1758), Myrsidea serini (Séguy, 1944) from Serinus serinus (Linnaeus, 1766), Myrsidea queleae Tendeiro, 1964 from Quelea quelea lathami (Smith, A., 1836), Myrsidea textoris Klockenhoff, 1984 from Ploceus cucullatus cucullatus (Müller, 1776), and Myrsidea viduae Tendeiro, 1993 from Vidua macroura (Pallas, 1764). Intraspecific morphometric variability, relative genetic divergence (based on a 379 bp portion of the mitochondrial COI gene and a 347 bp portion of the nuclear EF-1α gene), geographical distribution, and host associations, including 8 new host records for these lice, are discussed. Taking into consideration these parameters we suggest that the only way to deal with these taxa is to follow concept of subspecies with the following taxa and their geographic distributon: Palearctic Region: M. q. quadrifasciata and M. q. serini, Neotropical Region: M. q. anoxanthi, M. q. argentina, M. q. darwini, Paleotropic Region: M. q. queleae, M. q. textoris and M. q. viduae.
Chewing louse, polyxenous, geographic distribution, host specificity, morphometry, parasite
Chewing lice are traditionally considered as highly host-specific ectoparasites. Lice infesting multiple unrelated hosts were long thought to constitute cryptic species, which resulted in the erection of new species, and even genera, based primarily on host relationships (
Myrsidea is the most speciose genus of chewing lice with more than 380 species. It is also a good example of highly host-specific lice, with 80% of species being monoxenous – restricted to one avian host species (
Recently we collected Myrsidea lice from Spinus magellanicus (Vieillot, 1805) from the family Fringillidae. This avian speices is documented as the type host of M. argentina (Kellogg, 1906), in Peru. Myrsidea argentina was described by
Our opinion was supported by our preliminary molecular data. A portion of the mitochondrial cytochrome oxidase I (COI) gene of Myrsidea from Spinus magellanicus from Peru and M. serini from Agelaoides badius badius (Vieillot, 1819) from the family Icteridae from Paraguay was sequenced and the divergence among these samples was only 6.6%. In comparison with other species of Neotropical Myrsidea with known sequences, these Myrsidea were highly differentiated from all others, with uncorrected p-distance exceeding 18.2% that is well over a limit of interspecific genetic diversity of amblyceran lice proposed at level of 12% by
Curiously, the closest to our sequence of Myrsidea from S. magellanicus was that of Myrsidea textoris Klockenhoff, 1984 ex Ploceus intermedius cabanisii (W.K.H. Peters, 1868) and Ploceus velatus tahatali Smith A., 1836 from the family Ploceidae from South Africa, with a p-distance of only 5.3%. The next closest sequence is of Myrsidea sp. ex Vidua macroura (Pallas, 1764) from the family Viduidae from Cameroon, with p-distance 7.7% (
On the basis of morphology of male genitalia within Myrsidea species,
The aims of this paper are to: 1) re-describe M. quadrifasciata; 2) designate a neotype for this species; 3) analyze the validity of all other louse species currently placed in the “serini species group”; 4) synonymize all other 9 species from this species group with M. quadrifasciata and designate 8 subspecies; 5) present new host records for M. quadrifasciata; and 6) summarize its geographical distribution.
We used the setal counting system for metanotal and tergal setae as recommended by
Since previous authors (
We were able to examine specimens of M. balati, M. quadrifasciata, M. queleae, M. serini, M. textoris, and M. viduae. For comparison to other species (M. anoxanthi, M. darwini, M. major), we used precise descriptions or redescriptions of these species by
For statistical analysis, we used the most variable data mentioned by
Principal Component Analyses (PCAs) were run to additionally examine morphological characteristics of male and female lice. The R package ggplot2 (
Sequences of a 379 base pair (bp) fragment of the mitochondrial cytochrome c oxidase I gene (COI) were obtained from Myrsidea sp. ex Spinus magellanicus from Peru (A/N KY113129), M. serini ex Agelaioides badius from Paraguay (A/N KY113130), Myrsidea sp. ex Microspingus melanoleucus (A/N MT526017), M. textoris ex Ploceus intermedius and Ploceus velatus from South Africa (A/N KF768813–KF768815), using methods described by
Sequences of a 347 bp fragment of the nuclear elongation factor 1-alpha (EF-1α) gene were obtained from Myrsidea sp. ex Spinus magellanicus from Peru (A/N MT515729), M. serini ex Agelaioides badius from Paraguay (A/N MT515731), Myrsidea sp. ex Microspingus melanoleucus (A/N MT515735), and Myrsidea sp. ex Sporophila nigricollis (A/N MT968994) using methods described by
In order to assess the genetic divergence within the M. quadrifasciata complex, uncorrected p-distances from each specimen was obtained for COI and EF-1α sequences, sequences of five species with lowest p-distances of COI obtained by BLASTing our sequences against GenBank (M. cf. bubalornithis Klockenhoff, 1984, M. seminuda Eichler, 1951, M. cf. textoris, Myrsidea sp. ex Vidua macroura, and Myrsidea sp. ex Linurgus olivaceus) and sequences of three species from Ploceidae (M. eisentrauti Klockenhoff, 1982, M. ledgeri Klockenhoff, 1984, and Myrsidea sp. ex Ploceus nigricollis) (see Table
In order to evaluate the position of M. quadrifasciata complex within Myrsidea, two phylogenetic analyses were performed: 1) analysis based on the COI gene fragment, and 2) analysis based on concatenated sequences of the COI gene fragment and the EF-1α gene fragment. To build the COI gene tree, we first downloaded all available Myrsidea sequences from the GenBank and subsequently utilized all the full-length sequences (379 bp), which were unique (except for M. nesomimi where only single representatives of each of the subspecies M. nesomimi borealis Palma and Price, 2010 and M. nesomimi nesomimi Palma and Price, 2010 were selected in order to keep the analysis presentable). The final alignment consisted of 186 sequences (including Dennyus hirundinis as an outgroup taxon for rooting) and 387 bp. For a list of utilized sequences, see Table S2 in the Supplementary material.
For the concatenated tree, we downloaded all available Myrsidea sequences from the GenBank database and subsequently included all available samples with both COI and EF-1α sequences. Pairs of sequences for each sample were concatenated and all unique concatenates were subsequently used to build the phylogenetic tree. The final alignment consisted of 64 sequences (including Dennyus hirundinis as an outgroup taxon for rooting) and 675 bp. For a list of utilized sequences, see Table S2 in the supplementary material.
For both phylogenetic analyses, we first used the Akaike information criterion (AIC) computed in MEGA 7.0.14 (
Psocodea Hennig, 1966: 187
Phthiraptera Haeckel, 1896: 703
Amblycera Kellogg, 1896a: 68
Menoponidae Mjöberg, 1910: 26
Myrsidea Waterston, 1915: 12
Menopon quadrifasciatum Piaget, 1880: 440, pl. XXXV, fig. 6. Type host: Passer domesticus (Linnaeus, 1758).
Myrsidea quadrifasciata (Piaget, 1880):
Menopon quadrifasciatum var. major Piaget, 1880: 441. Type host: Plectrophenax nivalis (Linnaeus, 1758). New synonymy.
Myrsidea major (Piaget, 1880):
Menopon argentinus Kellogg, 1906: 49, pl. II, fig. 7. Type host: Spinus magellanicus (Vieillot, 1805). New synonymy.
Myrsidea argentina (Kellogg, 1906):
Menopon serini Séguy, 1944: 80, fig. 84. Type host: Serinus serinus (Linnaeus, 1766). New synonymy.
Myrsidea serini (Séguy, 1944):
Liquidea serini (Séguy, 1944): Złotorzycka (1964: 169, 176).
Myrsidea queleae Tendeiro, 1964: 182, photos 11–16. Type host: Quelea quelea lathami (Smith A., 1836). New synonymy.
Myrsidea queleae Tendeiro, 1964:
Myrsidea balati Macháček, 1977a: 1, figs 1a, b, 4, 7–8. Type host: Passer montanus (Linnaeus, 1758). New synonymy.
Myrsidea balati Macháček, 1977:
Myrsidea textoris Klockenhoff, 1984b: 270, figs 1–3, 10a, 11a, b. Type host: Ploceus cucullatus cucullatus (Müller, 1776). New synonymy.
Myrsidea textoris Klockenhoff, 1984b: Lindholm et al. (1998: 147);
Myrsidea viduae Tendeiro, 1993: 57, figs 2, 4, 6. Type host: Vidua macroura (Pallas, 1764). New synonymy.
Myrsidea viduae Tendeiro, 1993:
Myrsidea anoxanthi Price and Dalgleish, 2007: 13, figs 40–44. Type host: Loxipasser anoxanthus (Gosse, 1847). New synonymy.
Myrsidea darwini Palma and Price, 2010: 136, figs 1–5. Type host: Geospiza fuliginosa Gould, 1837. New synonymy.
Passer domesticus (Linnaeus, 1758) (Passeridae).
Unknown (most likely Netherlands).
In both sexes showing the characteristics of the “M. serini-Artengruppe” (
Male genital sac sclerites of Myrsidea quadrifasciata. 4–5: M. q. quadrifasciata ex Passer domesticus. 6–7: M. q. quadrifasciata ex Passer montanus. 8: M. q. argentina ex Agelaoides badius from Paraguay. 9–11: M. q. argentina according to
The following overall description is based on a large number of specimens from different hosts. Data for the most important morphometric characteristics for specimens according to their hosts are presented in supplement Tables S3b–S12. For better orientation and to avoid repetition of lists of hosts in the following text we refer to Myrsidea from particular hosts under their previous names in quotation marks, for example, “M. serini”, “M. textoris”, etc. (for specification see Table S1).
To evaluate the status of “M. argentina” we also examined available nymphs of 3rd instar: 1) two nymphs from Spinus magellanicus – type host of “M. argentina”, and 2) one nymph from Passer montanus – host of M. quadrifasciata. These nymphs differ from previous descriptions of “M. argentina” by
FEMALE
(n=167) (as in Fig.
MALE
(n=90) (as in Fig.
THIRD INSTAR NYMPH . Marginal seta of metanotum 7 (4/6). Tergocentral setae of abdomen: I, 7–10 (10/8–9); II, 8–9 (11/8); III, 8 (11/8–9); IV, 8 (11/8–9); V, 6–7 (10/6–7); VI, 6 (10/6–7); VII, 5–6 (9/6); VIII, 4–5 (4/4). Number of setae of dorsal anal fringe, 16–21 (cca 15/15). Dimensions: HL, 0.25–0.29 (0.27/0.28–0.30); TW, 0.35–0.36 (0.35/0.39–0.40); TL, 1.20–1.29 (1.20/1.40–1.41).
Ex Passer domesticus (Passeridae): 1♂ (designated as a neotype), England: Cheshire, Great Budworth, 5.xii.1934, A.W. Boyd leg. (NHML: B.M.1955–616); 2♂, 2♀, USA: Mississippi, Tibbee, 15.iii.1936, E.W. Stafford leg. (KCEM: 8170, 8172–74); 1♂, 1♀, USA: Hawaii, Honolulu, 8.ii.–8.iii.1947, J. Alicata leg. (USNM: Lot 47-4795, vial 2). — Ex Passer montanus (Passeridae): 1♀ (paratype of M. balati), Czech Republic, Nesyt, 9.xi.1973, P. Macháček leg. (ZFMK: 1986/15), 1♂, Czech Republic, Jinačovice (49°15′N 16°31′E), 13.i.2006, O. Sychra and I. Literak leg. (MMBC), 1♀, Czech Republic, Moravské Knínice (49°17′N 16°29′E), 8.ii.2009, O. Sychra and I. Literak leg. (MMBC), 1♀, Czech Republic, Kardašova Řečice, 19.vii.1938, K. Pfleger leg. (SNMB); 1♀, Hungary, Nagykanizsa, 28.vi.1952, Balát coll. (MMBC: B185), 1♀, Hungary, Bajcza (Zala m.), 19.iv.1953, Balát coll. (MMBC: C579); 1♀, 1 nymph, Slovakia, Gabčíkovo, 22.vii.1953, Balát coll. (MMBC: 1380), 2♂, 1♀, Slovakia, Gbelce (47°51′N 18°30′E), 10.vii.2019, O. Sychra and L. Oslejskova leg.; 3♂, 3♀, Thailand, San Sai, Ban Pong, 16.ii.1962, Kitti Thonglongya leg. (KCEM: 8183–85); 1♀, W. Java, Bogor, 8.xi.1968 (KCEM: 9E 0414); 2♀, no data (NHML: 840). — Ex Agelaius phoeniceus (Linnaeus, 1766) (Icteridae): 9♀, 3♂, USA: South Carolina, Charleston, 1934, 27.iii.1933, H.S. Peters leg. (USNM: Bish. 1934 #20711). — Ex Agelaoides badius badius (Vieillot, 1819) (Icteridae): 1♀, 4♂, Paraguay, Los Tres Gigantes Biological Station in the Pantanal (20°04′S 50°09′W), 6.ix.2012, I. Literak leg. (MMBC: PG357). — Ex Emberiza citrinella caliginosa Clancey, 1940 (Emberizidae): 1♀, 1♂, New Zealand, Raoul I., Kermadec Is., 11.xii.1972; J. Ireland leg., R.L.C. Pilgram Collection (MONZ). — Ex Euplectes franciscanus (Isert, 1789) (Ploceidae): 2♂, 2♀, Senegal, Niokolo Koba National Park, Simenti (13°02′N 13°18′W), 8.ii.2005, P. Prochazka leg. (MMBC). — Ex Euplectes jacksoni (Sharpe, 1891) (Ploceidae): 1♂, 3♀, Kenya, i.1936, Meinertzhagen coll. (NHML: No.6081). — Ex Euplectes orix (Linnaeus, 1758) (Ploceidae): 2♀, South Africa, Pietermaritzburg, Scottsville (29°39′S 30°23′E), 7. and 19.ii.1994, A. Lindholm leg. (slide no. 57A, 106A). — Ex Euplectes progne delamerei (Shelley, 1903) (Ploceidae): 2♂, Kenya, iii.1936, Meinertzhagen coll. (NHML: No.7462); 1♂, 3♀, Kenya, ii.1936, Meinertzhagen coll. (NHML: No.6715). — Ex Foudia madagascariensis (Linnaeus, 1766) (Ploceidae): 1♂, 2♀, Madagascar, Diego Suarez, 1921, G. Melow Coll. (NHML: 1921–200). — Ex Passer luteus (Lichtenstein, M.H.C., 1823) (Passeridae): 3♀, Senegal, Matam (15°37′N 13°20′W), 6.ix.2007, I. Literak and M. Capek leg. (MMBC). — Ex Ploceus cucullatus cucullatus (Statius Müller, 1776) (Ploceidae): 1♂, 3♀, Senegal, Kaolack (14°09′N 16°06′W), 7.ix.2007, I. Literak and M. Capek leg. (MMBC). — Ex Microspingus melanoleucus (d‘Orbigny and Lafresnaye, 1837) (Thraupidae): 1♀, Paraguay, Los Tres Gigantes Biological Station in the Pantanal (20°04′S 50°09′W), 6.ix.2012, I. Literak leg. (MMBC: PG359). — Ex Ploceus cucullatus nigriceps (Layard, 1867) (Ploceidae): 1♂, Mozambique, Zambue, Tete District, 3.ix.1964, A.L.Moore leg. (KCEM: A36). — Ex Ploceus nigricollis brachypterus Swainson, 1837 (Ploceidae): 1♂, 1♀, Cameroon, Yaounde, 1955, J. Mouchet (NHML: B.M.1955–737). — Ex Ploceus philippinus (Linnaeus, 1766) (Ploceidae): 1♂, 5♀, 1 nymph, India, Deccan, ii.1937, Meinertzhagen coll. (NHML: No.8615–17); 2♀, India, Daulatabad, Maharastra, 25.vi.1969, (KCEM: S.No.XE–363, XE–193, AB–24042); 1♀, India, Daulatabad, Aurangabad, 20.vii.1968, (KCEM: 9E 0250, A81348); 1♀, Thailand, Doi Pha Hom Pok Chiengmai 22.xii.1965, (KCEM: MAPS–3658). — Ex Ploceus velatus tahatali A. Smith, 1836 (Ploceidae): 1♂, South Africa, Limpopo province, Polokwane Game Reserve (23°58′S 29°28′E), 11.ii.2012, A. Halajian leg. (MMBC). — Ex Quelia cardinalis (Hartlaub, 1880) (Ploceidae): 1♂ (paratype of M. queleae), Bechuanaland (now Botswana), Mababe, 6.x.1952, F. Zumpt leg. (NHML: B.M. 1959–273). — Ex Quelea quelea aethiopica (Sundevall, 1850) (Ploceidae): 1♂, 1♀, Sudan, May 1936, Meinertzhagen coll. (NHML: No.7836). — Ex Quelea quelea lathami (Ploceidae): 1♂, Southern Rhodesia (now Zimbabwe), Matopos, 30.iii.1952 (NHML: B.M.1980–40, coll.691); 1♂, 1♀, Transvaal (now South Africa), Nr. Komatipoort, 18.i.1961, F. Zumpt leg. (NHML: B.M.1965–526); 4♂, 3♀, South Africa, Limpopo province, De Loskop (23°30′S 29°18′E), 7.xii.2012, Halajian leg. (MMBC). — Ex Quelea quelea quelea (Linnaeus, 1758) (Ploceidae): 1♂, 1♀, North Cameroon, Marona, J.Mouchet leg. (NHML); 2♂, 2♀, Senegal, Matam (15°37′N 13°20′W), 6.ix.2007, I. Literak and M. Capek leg. (MMBC). — Ex Serinus canaria (Linnaeus, 1758)–captive bird (Fringillidae): 1♀, 1♂, New Zealand, Christchurch, 20.xii.1944, R.L.C. Pilgram Collection (MONZ). — Ex Spinus magellanicus (Fringillidae): 4♀, 2♂, 2 nymphs, Peru, Cascay, Huanuco (9°50’S 76°08’W), 20. and 22.viii.2011, I. Literak leg (MMBC: O. Sychra PE16–19). — Ex Sporophila nigricollis (Vieillot, 1823) (Thraupidae): 1♂, Peru, Cascay, Huanuco (9°50’S 76°08’W), 21.viii.2011, I. Literak leg (MMBC: O. Sychra PE20). — Ex Vidua macroura (Viduidae): 2♀, São Tomé and Príncipe, Missão Zoológica a São Tomé, loc. 41, São João dos Angolares (MZUL: 23/6/984).
We were able to examine slide no. 840 mentioned by
There are few reports about the occurrence of M. quadrifasciata on P. domesticus and P. montanus (see Table
Summary of published records of examined sparrows and collected Myrsidea quadrifasciata quadrifasciata from Passer domesticus and Passer montanus within and out of their native range. –– Abbreviations: E=number of examined birds; P=number of parasitised birds; %=prevalence; MA=mean abundance; ?=not mentioned.
Host / Country | E | P | % | MA | Number of collected lice | Reference |
Passer domesticus | ||||||
Azerbaijan | 514 | 21 | 4.1 | 0.078 | 40 |
|
Belarus | 93 | 0 | 0 | 0 | – | Zhuk and Nikalaeva (1987) |
Bulgaria | 118 | 1 | 0.8 | 0.008 | 1 ♀ |
|
Czech Republic | 436 | 1 | 0.2 | 0.002 | 1 ♂ | Macháček (1977a) |
Czech Republic | 86 | 0 | 0 | 0 | – | present study |
England | 473 | 0 | 0 | 0 | – |
|
England | 237 | 0 | 0 | 0 | – |
|
India | 100 | 20 | 20 | ? | Range 2–28 lice per bird |
|
Iran | 9 | 0 | 0 | 0 | – |
|
Pakistan | 129 | 39 | 30.2 | 0.66 | 85 |
|
Romania | 492 | 0 | 0 | 0 | – |
|
Turkey | 22 | 0 | 0 | 0 | – |
|
TOTAL (within native range) | 2709 | 82 | 3.0 | ? | ||
Canada, Manitoba | 455 | 0 | 0 | 0 | – | Galloway (pers. comm.) |
Panama | 58 | 0 | 0 | 0 | – |
|
USA, Indiana | 300 | 0 | 0 | 0 | – |
|
USA, Kansas | 567 | 0 | 0 | 0 | – |
|
USA, Kentucky | 77 | 0 | 0 | 0 | – |
|
USA, Massachusetts | 34 | 0 | 0 | 0 | – |
|
USA, New Hampshire | 44 | 0 | 0 | 0 | – |
|
USA, New Jersey | 62 | 0 | 0 | 0 | – |
|
USA, Oklahoma | 127 | 0 | 0 | 0 | – |
|
USA, Wisconsin | 391 | 0 | 0 | 0 | – | Woodmann and Dickie (1954) |
TOTAL (out of native range) | 1660 | 0 | 0 | 0 | ||
Passer montanus | ||||||
Belarus | 235 | 0 | 0 | 0 | – | Zhuk and Nikalaeva (1987) |
Czech Republic | 433 | 2 | 0.5 | 0.021 | 2♂, 2♀, 5 nymphs | Macháček (1977a) |
Czech Republic | 15 | 2 | 13 | 0.133 | 1♂, 1♀ | present study |
Iran | 8 | 0 | 0 | 0 | – |
|
Thailand | 140 | 70 | 50 | ? | ? |
|
TOTAL (within the native range) | 831 | 74 | 9.0 | ? |
Within the M. quadrifasciata complex, we found genetic divergences of 0.0–6.6% among the obtained sequences of COI from six Myrsidea samples examined in this study (Table
Phylogenetic tree of the Myrsidea species based on concatenated partial COI and EF-1α sequences. The tree was inferred using the maximum likelihood method based on the GTR+G+I model. The tree with the highest log likelihood is shown. Bootstrap support is shown next to the branches (values < 50% not shown). The tree is drawn to scale, with branch lengths in proportion to expected number of substitutions per site, as represented by the scale bar. Samples of M. quadrifasciata discussed in the present paper are in bold type. –– Colours: green – samples from Ethiopian Region; red – samples from Neotropical Region; blue – samples from Nearctic Region.
Genetic distance between available specimens of Myrsidea quadrifasciata (= M. q., in bold type) and six related species; upper right and lower left distance collected from COI and EF-1α partial gene pairwise comparisons. GenBank numbers for COI and EF-1α, respectively: 1) KY113129, MT515729; 2) KY113130, MT515731; 3) MT526017, MT515735; 4) COI not available, MT968994; 5) DQ887256, DQ887220; 6) DQ887257; DQ887221; 7) KF768813, EF-1α not available; 8) KF768814, EF-1α not available; 9) KF768815, EF-1α not available; 10) MG682397, EF-1α not available; 11) MG682394, EF-1α not available; 12) MG765498, EF-1α not available; 13) FJ171275, FJ171301; 14) KY359403, KY359392; 15) AF545733, AF320428; 16) AF545731, AF320429. * denotes amblycerans examined in this study.
(sub)Species | EF-1α | ||||||||||||||||
1) | 2) | 3) | 4) | 5) | 6) | 7) | 8) | 9) | 10) | 11) | 12) | 13) | 14) | 15) | 16) | ||
COI | *1) M. q. argentina ex Spinus magellanicus | 0.0 | 0.0 | 0.0 | 0.0 | 0.3 | N/A | N/A | N/A | N/A | N/A | N/A | 7.8 | 5.5 | 8.1 | 7.4 | |
*2) M. q. argentina ex Agelaoides badius | 6.6 | 0.0 | 0.0 | 0.0 | 0.3 | N/A | N/A | N/A | N/A | N/A | N/A | 7.8 | 5.5 | 8.1 | 7.4 | ||
*3) M. q. argentina ex Microspingus melanoleucus | 5.5 | 5.8 | 0.0 | 0.0 | 0.3 | N/A | N/A | N/A | N/A | N/A | N/A | 7.8 | 5.5 | 8.1 | 7.4 | ||
*4) M. q. anoxanthi ex Sporophila nigricollis | N/A | N/A | N/A | 0.0 | 0.3 | N/A | N/A | N/A | N/A | N/A | N/A | 7.8 | 5.5 | 8.1 | 7.4 | ||
5) M. q. viduae ex Vidua macroura | 7.7 | 7.4 | 6.6 | N/A | 0.3 | N/A | N/A | N/A | N/A | N/A | N/A | 7.8 | 5.5 | 8.1 | 7.4 | ||
6) M. q. viduae ex Vidua macroura | 7.7 | 7.9 | 6.6 | N/A | 0.5 | N/A | N/A | N/A | N/A | N/A | N/A | 8.1 | 5.8 | 8.4 | 7.7 | ||
*7) M. q. textoris ex Ploceus intermedius | 5.3 | 5.6 | 6.1 | N/A | 7.7 | 8.2 | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | ||
*8) M. q. textoris ex Ploceus velatus | 5.6 | 5.8 | 6.4 | N/A | 7.9 | 8.5 | 0.3 | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | ||
*9) M. q. textoris ex Ploceus velatus | 5.3 | 5.6 | 6.1 | N/A | 7.7 | 8.2 | 0.0 | 0.3 | N/A | N/A | N/A | N/A | N/A | N/A | N/A | ||
10) M. q. textoris ex Ploceus ocularis | 6.9 | 6.6 | 7.7 | N/A | 8.7 | 9.3 | 1.6 | 1.9 | 1.6 | N/A | N/A | N/A | N/A | N/A | N/A | ||
11) M. cf. bubalornithis ex Bubalornis niger | 14.3 | 15.6 | 16.4 | N/A | 16.9 | 16.9 | 16.1 | 16.4 | 16.1 | 16.4 | N/A | N/A | N/A | N/A | N/A | ||
12) Myrsidea sp. ex Linurgus olivaceus | 16.1 | 15.3 | 13.7 | N/A | 14.7 | 14.7 | 14.5 | 14.8 | 14.5 | 15.0 | 18.2 | N/A | N/A | N/A | N/A | ||
13) Myrsidea seminuda ex Thraupis palmarum | 18.2 | 18.5 | 19.0 | N/A | 19.8 | 19.8 | 17.7 | 17.5 | 17.7 | 18.3 | 20.8 | 19.5 | 4.9 | 7.5 | 6.6 | ||
14) Myrsidea sp. ex Ploceus nigricollis | 22.2 | 21.2 | 21.2 | N/A | 21.7 | 21.7 | 20.6 | 20.4 | 20.6 | 21.2 | 21.4 | 18.7 | 20.9 | 7.0 | 5.7 | ||
15) Myrsidea ledgeri ex Philetairus socius | 23.2 | 24.0 | 23.0 | N/A | 21.4 | 21.4 | 22.8 | 23.0 | 22.8 | 23.5 | 22.4 | 23.7 | 26.4 | 22.2 | 8.0 | ||
16) Myrsidea eisentrauti ex Sporopipes squamifrons | 24.0 | 22.4 | 23.2 | N/A | 22.4 | 22.4 | 22.5 | 22.2 | 22.5 | 23.5 | 23.8 | 23.2 | 21.1 | 23.0 | 24.5 |
Principal Component Analysis (Fig.
Passer domesticus, the type host of M. quadrifasciata, is a widespread species. Its native range includes the Palearctic and Oriental Regions, but it was also introduced to the Nearctic and Neotropical Regions and the southern parts of the Afrotropical and Australasian Regions (
Myrsidea quadrifasciata is prevalent in Asia with a prevalence 20–50% (Table
“Myrsidea anoxanthi”.
“Myrsidea argentina”. Myrsidea argentina was described by
“Myrsidea balati”. Myrsidea balati was described on the basis of two males and two females found on two of 434 examined Passer montanus by Macháček (1977a), who was able to compare them with one male of Myrsidea quadrifasciata that he found on one of 436 examined Passer domesticus in the Czech Republic. Unfortunately, the slides with holotype male (No. 2–320a) and allotype female (No. 2–320c) are probably lost (Vladimir Jansky, Slovak National Museum, Bratislava, Slovakia, pers. comm. 2017). There is only the second and last paratype male available in the collection of ZFMK.
Contrary to ischnoceran lice, where Macháček (1977b) correctly suggested that both species of sparrows share the same species of lice, Brueelia cyclothorax (Burmeister, 1838), Philopterus fringillae (Scopoli, 1772) and Sturnidoecus ruficeps (Nitzsch, 1866), in the case of Myrsidea, unfortunately he was wrong. As main diagnostic characteristics of M. balati, he used the ratio of lengths of setae in asters, head ratio and total length, and he compared only three males. When we compared our examined specimens, we found that all aforementioned characteristics of Myrsidea from both species of sparrows overlap. Since all other characters are almost identical (Tables S3–S12), we place M. balati as a junior synonym of M. quadrifasciata. It is also in accordance with
“Myrsidea darwini”.
When we compared morphometric characteristics of these species according to their original descriptions and all examined specimens, there are no more significant differences either in number of abdominal setae of both sexes or in the relative length of the postspiracular setae (see Tables S3–S12). Slight differences of the male genital sac sclerites mentioned by
“Myrsidea major”.
“Myrsidea queleae”. This species was described by
“Myrsidea serini”. This species was described by Séguy (1944) from Serinus serinus from the family Fringillidae from France. Later, it was redescribed by
“Myrsidea textoris”. This species was described by
“Myrsidea viduae”. This species was described on the basis of only two females found on Vidua macroura from Sao Tomé e Principe by
Myrsidea from Microspingus melanoleucus. We found only one female of Myrsidea on this host in Paraguay (see material examined). At the same day when we collected this female on Microspingus melanoleucus (bird no. PG359), we also examined one Agelaoides badius (bird no. PG357) with a few Myrsidea (reported as “M. serini” by
In cases where there is a larger number of examined females, such as for M. quadrifasciata from Passer montanus (n=11) or “M. serini” reported by
On the other hand, there are “M. serini” from Agelasticus thilius petersii from Argentina and “M. queleae” from Africa with their numbers of tergal setae at the upper limit of the range (Tables S3 and S4). Moreover, females of “M. serini” from Agelasticus thilius petersii differ from all examined specimens by 8 setae on tergite VIII (vs. 3–6 setae; Table S3). Due to this fact, we have doubts as to whether these individuals really represent the species under consideration. More specimens from this host are needed to resolve this problem.
In the case of males, the highest numbers of tergal setae are recorded mainly on males of “M. queleae” and “M. textoris” from Africa. The most conspicuous differences are visible on tergite VIII: while specimens from Neotropical (“M. anoxanthi”, “M. darwini” and “M. serini”) and Palearctic (“M. balati” and “M. quadrifasciata”) have 4–8 setae (one exception is again “M. serini” from Agelasticus thilius petersii with 11 setae), specimens from Africa (“M. queleae” and “M. textoris”) have 8–14 setae (Table S4). Conversely, “M. serini” from Palearctic shows wide range of number of setae (6–12 setae) that overlap range of setae found on both aforementioned examples. Unfortunately,
When we compare sternal chaetotaxy, we see a similar pattern as for tergites: 1) Neotropical specimens lie at the lower limit of the range of these setae; 2) African specimens, in this case including specimens from sparrows (“M. balati” and “M. quadrifasciata”), lie at the upper limit of the range of these setae; and 3) cases where there are larger numbers of examined specimens, i.e. “M. serini” reported by
Postspiracular setae show the same pattern in their ratio of lengths, with high variability in the lengths of these setae on a particular segment. In general, there are long to extremely long postspiracular setae on II, IV, VII and VIII and shorter with variable length setae on I, and shortest on III, V and VI (Tables S9 and S10).
Concerning different body sizes, in general, “M. anoxanthi” and “M. viduae” are represented by the smallest individuals (for example, TW of females 0.34–0.37 and TW of males 0.33–0.34), while Myrsidea from the Icteridae are represented by the largest ones (for example TW of females 0.44–0.46 and TW of males 0.40–0.42). Similarly, as in the case of setal counts, “M. serini” reported by
Observing the PCA plots for PC1 and PC2 and the PC1 and PC3 revealed the overlapping of all examined groups of Myrsidea, supporting that all analysed individuals of M. quadrifasciata complex form one morphological group with a few outliers.
Taking into consideration all these parameters, host associations and geographic distribution, we suggest that the only way to deal with these taxa is to follow the concept of subspecies.
Because for most Myrsidea species, only a relatively short sequence of the COI gene is available, all conclusions inferred from the phylogenetic analyses are necessarily limited; no deeper phylogenetic conclusions can be reached and we can not speculate about the definitive position of the M. quadrifasciata complex in context of the genus Myrsidea. This necessary caution is further supported by relatively low bootstrap supports in the majority of tree branches (see Figs
The subspecies concept we are using here is accepted for other chewing lice, for example lice from the genera Gyropus (Gyropidae), Actornithophilus, Dennyus, Menacanthus (Menoponidae), Lunaceps, Saemundssonia (Philopteridae), Geomydoecus, Procaviphilus (Trichodectidae) (
We propose the following subspecies (a list of their hosts and their geographic distributions is given in Table
Palearctic Region:
M. q. quadrifasciata (Piaget, 1880) comb. nov.
M. q. serini (Séguy, 1944) comb. nov.
Paleotropic Region:
M. q. queleae Tendeiro, 1964 comb. nov.
M. q. textoris Klockenhoff, 1984 comb. nov.
M. q. viduae Tendeiro, 1993 comb. nov.
Neotropical Region:
M. q. anoxanthi Price and Dalgleish, 2007 comb. nov.
M. q. argentina (Kellogg, 1906) comb. nov.
M. q. darwini Palma and Price, 2010 comb. nov.
List of hosts of Myrsidea quadrifasciata and their geographic distribution.
Hosts family Host species |
Location | References |
---|---|---|
Myrsidea quadrifasciata anoxanthi | ||
Thraupidae | ||
Loxipasser anoxanthus (Gosse, 1847) | Jamaica |
|
Sporophila minuta (Linnaeus, 1758) | Venezuela |
|
Sporophila nigricollis (Vieillot, 1823) | Peru | present study |
Myrsidea quadrifasciata argentina | ||
Fringillidae | ||
Spinus barbatus (Molina, 1782) | Chile |
|
Spinus magellanicus (Vieillot, 1805) | Peru | present study |
Icteridae | ||
Agelaioides badius badius (Vieillot, 1819) | Argentina |
|
<< „ „ „ >> | Paraguay |
|
Agelasticus thilius petersii (Laubmann, 1934) | Argentina |
|
Agelaius phoeniceus (Linnaeus, 1766) | USA: South Carolina | present study |
Thraupidae | ||
Microspingus melanoleucus (d‘Orbigny and Lafresnaye, 1837) | Paraguay | present study |
Myrsidea quadrifasciata darwini | ||
Thraupidae | ||
Camarhynchus psittacula Gould, 1837 | Galápagos Islands |
|
Geospiza fuliginosa Gould, 1837 | Galápagos Islands |
|
Geospiza magnirostris Gould, 1837 | Galápagos Islands |
|
Myrsidea quadrifasciata quadrifasciata | ||
Emberizidae | ||
Plectrophenax nivalis (Linnaeus, 1758) | no location data |
|
Passeridae | ||
Passer domesticus (Linnaeus, 1758) | Netherlands? |
|
<< „ „ „ >> | Azerbaijan |
|
<< „ „ „ >> | Bulgaria |
|
<< „ „ „ >> | Czech Republic | Macháček (1977a) |
<< „ „ „ >> | England |
|
<< „ „ „ >> | France | Séguy (1944) |
<< „ „ „ >> | Germany |
|
<< „ „ „ >> | Hungary? | Fauna Europaea (www.fauna-eu.org) - but not confirmed by |
<< „ „ „ >> | Italy |
|
<< „ „ „ >> | India |
|
<< „ „ „ >> | Pakistan |
|
<< „ „ „ >> | Sweden | present study (Daniel Gustafsson, pers. comm.) |
<< „ „ „ >> | USA, Mississippi | present study |
<< „ „ „ >> | USA, Hawaii |
|
Passer montanus (Linnaeus, 1758) | Czech Republic | Macháček (1977a), present study |
<< „ „ „ >> | Bulgaria |
|
<< „ „ „ >> | Hungary | present study |
<< „ „ „ >> | Slovakia | present study |
<< „ „ „ >> | Romania |
|
<< „ „ „ >> | Thailand |
|
<< „ „ „ >> | W. Java | present study |
Myrsidea quadrifasciata queleae | ||
Ploceidae | ||
Quelea cardinalis (Hartlaub, 1880) | Botswana |
|
Quelea quelea aethiopica (Sundevall, 1850) | Kenya, Sudan, |
|
Quelea quelea quelea (Linnaeus, 1758) | Senegal |
|
<< „ „ „ >> | Cameroon | present study |
Quelea quelea lathami (Smith) | Congo, South Africa, Zambia |
|
Passer luteus (Lichtenstein M.H.C., 1823)* – probably stragglers | Senegal | present study |
Myrsidea quadrifasciata serini | ||
Emberizidae | ||
Emberiza citrinella caliginosa Clancey, 1940 | New Zealand |
|
Fringillidae | ||
Carduelis carduelis britannica (Hartert, 1903) | New Zealand |
|
Carduelis carduelis parva Tschusi, 1901 | Spain |
|
Chloris chloris chloris (Linnaeus, 1758) | New Zealand |
|
Serinus canaria (Linnaeus, 1758) – domesticated form | England, New Zealand |
|
<< „ „ „ >> | Netherlands | RMNH.INS.UT.479; No. B01/1887; 12-09-2001 (parasites_collection_utrecht_naturalis.xls) |
<< „ „ „ >> | Czech Republic | present study |
Serinus serinus (Linnaeus, 1766) | France | Séguy (1944) |
<< „ „ „ >> | Morocco |
|
<< „ „ „ >> | Romania |
|
Myrsidea quadrifasciata textoris | ||
Ploceidae | ||
Euplectes franciscanus (Isert, 1789) | Senegal | present study |
Euplectes jacksoni (Sharpe, 1891) | Kenya | present study |
Euplectes orix (Linnaeus, 1758) | South Africa | Lindholm et al. (1998), present study |
Euplectes progne delamerei (Shelley, 1903) | Kenya | present study |
Foudia madagascariensis (Linnaeus, 1766) | Madagascar | present study |
Ploceus capensis (Linnaeus, 1766) | South Africa, Mozambique |
|
Ploceus cucullatus cucullatus (Müller, 1776) | Ghana, Senegal |
present study |
Ploceus cucullatus nigriceps (Layard, 1867) | South Africa Mozambique |
present study |
Ploceus cucullatus spilonotus Vigors, 1831 | South Africa |
|
Ploceus intermedius cabanisii (W.K.H. Peters, 1868) | South Africa | Linholm et al. (1998), |
Ploceus nigricollis brachypterus Swainson, 1837 | Cameroon | present study |
Ploceus ocularis A. Smith, 1828 | South Africa |
|
Ploceus philippinus (Linnaeus, 1766) | India, Thailand | present study |
Ploceus velatus tahatali A. Smith, 1836 | South Africa |
|
Ploceus velatus velatus (Vieillot, 1819) | South Africa, Botswana |
|
Myrsidea quadrifasciata viduae | ||
Viduidae | ||
Vidua macroura (Pallas, 1764) | Sao Tomé e Príncipe |
|
<< „ „ „ >> | Cameroon | Balakrishnan and Sorenson (2006) |
Our results revealed an interesting case of a cosmopolitan, polyxenous species of Myrsidea. Myrsidea quadrifasciata is unique within the genus that primarily includes, according to our knowledge, highly host-specific lice. This is similar to the case of Menacanthus eurysternus (Burmeister, 1838), another widespread species closely related to host-specific Menacanthus species. Despite the fact that this cosmopolitan host generalist has been recorded from almost 170 species of passerines belonging to 20 families, it possesses a relatively low level of differentiation, with sequences (COI and EF-1α) differing only in approximately 4% of nucleotide positions (
In our study, we demonstrated the importance of a comprehensive approach in taxonomy of such a large genus as Myrsidea. Since the only practical manner to deal with this genus was, and still it is, to treat lice from each host family as a unit it is easy to overlook similarity of Myrsidea parasitizing hosts from different families and regions. We expect that more complex review not only in this genus, but other genera of lice, will reveal additional similar cases.
We would like to thank everyone who helped us with field and laboratory work, especially to: Bernardo Calvo Rodríguez from Costa Rica; Alberto Andrés Velásquez Castillo from Honduras; Sebastian Hector Franco Ibarrola from Paraguay; and Jorge Manuel Cárdenas-Callirgos from Perú, Ali Halajian from South Africa, Petr Koubek, Petr Procházka, Miroslav Capek from the Czech Republic. Our special thanks go to the following persons and institutions for the loan of specimens used in this study: P. A. Brown (Natural History Museum, London, U.K.), Donald C. Arnold (K.C. Emerson Entomology Museum, Oklahoma State University, Stillwater, Oklahoma, USA), Dirk Rohwedder (Zoological Research Museum Alexander Koenig, Bonn, Germany), Ricardo L. Palma (Museum of New Zealand Te Papa Tongarewa, Wellington, New Zealand), Alexandra Marcal (Museu Bocage, Museo Zoologico da Universidade de Lisboa, Lisboa, Portugal), Anna Lindholm (Department of Zoology, Cambridge, U.K.), Igor Malenovsky (Moravian Museum, Brno, Czech Republic), Vladimir Jansky (Slovak National Museum, Bratislava, Slovakia), Patricia Gentili-Poole (National Museum of Natural History, Smithsonian Institution, Washington, D.C., USA). We are also grateful to three reviewers who provided helpful comments and suggestions to improve this paper. This work was supported by the project FVHE-Široký-2021ITA26 from the University of Veterinary Sciences, Brno, Czech Republic.
File 1
Data type: .xlsx
Explanation note: Table S1. List of hosts of Myrsidea quadrifasciata, their geographic distribution, body size (cm) and body mass (g) with data about louse female temple width (TW), total length (TL), and total number of tergal setae.
File 2
Data type: .xlsx
Explanation note: Table S2. List of COI and EF-1α sequences included in the phylogenetic analyses in this study. 64 samples with A/Ns for both markers were included in the concatenated tree; 186 samples, i.e. all samples minus four noted as „concatenated tree only“, were included in the COI tree.
File 3
Data type: .docx
Explanation note: Tables S3–S16. Morphometric characteristics: number of setae on gula, metanotum, metasternal plate and tergites of female of Myrsidea quadrifasciata from different hosts (T=tergites). — Table S4. Morphometric characteristics: number of setae on gula, metanotum, metasternal plate and tergites of male of Myrsidea quadrifasciata from different hosts (T=tergites). — Table S5. Morphometric characteristics: number of setae on sternites and anal fringe of female of Myrsidea quadrifasciata from different hosts (S=sternites; marg.=marginal; m.a.=medioanterior; AFD=anal fringe dorsal; AFV=anal fringe ventral). — Table S6. Morphometric characteristics: number of setae on sternites and ventral terminalia of male of Myrsidea quadrifasciata from different hosts (S=sternites; marg.=marginal; m.a.=medioanterior). — Table S7. Morphometric characteristics: number of setae on pleurites of female of Myrsidea quadrifasciata from different hosts (P=pleurites). — Table S8. Morphometric characteristics: number of setae on pleurites of male of Myrsidea quadrifasciata from different hosts (P=pleurites). — Table S9. Morphometric characteristics: length of dorsal head setae 10, 11 and postspiracular setae (in mm) of female of Myrsidea quadrifasciata from different hosts (DHS=dorsal head seta; PsS=postspiracular seta). — Table S10. Morphometric characteristics: length of dorsal head setae 10, 11 and postspiracular setae (in mm) of male of Myrsidea quadrifasciata from different hosts (DHS=dorsal head seta; PsS=postspiracular seta). — Table S11. Morphometric characteristics: dimensions (in mm) of female of Myrsidea quadrifasciata from different hosts (TW=temple width; POW=preocular width; HL=head length at midline; PW=prothorax width; MW=metathorax width; AWIV=abdomen width at level of segment IV; ANW=anus width; TL=total length). — Table S12. Morphometric characteristics: dimensions (in mm) of male of Myrsidea quadrifasciata from different hosts (TW=temple width; POW=preocular width; HL=head length at midline; PW=prothorax width; MW=metathorax width; AWIV=abdomen width at level of segment IV; GW=male genitalia width; GL=male genitalia length; ParL=paramere length; GSL=genital sac sclerite length.; TL=total length). — Table S13. Comparison of morphometric characteristics of female of Myrsidea quadrifasciata from different subspecies. –– Abbreviations: M=metanotal posterior setae; S=sternal setae; T=tergal setae. –– Symbols: After each comparison of our data with those mentioned by
File 4
Data type: .pdf
Explanation note: Figure S1. Phylogenetic tree of the Myrsidea species based on partial COI sequences. The tree was inferred using the maximum likelihood method based on the GTR+G+I model. The tree with the highest log likelihood is shown. Bootstrap support is shown next to the branches (values < 50% not shown). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Samples of M. quadrifasciata discussed in the present paper are in bold type. –– Colours: blue – samples from Nearctic Region; light blue – samples from Palearctic Region; green – samples from Ethiopian Region; red – samples from Neotropical Region; violet – samples from Oriental Region.